Serum amyloid A is a growth factor for 3T3-L1 adipocytes, inhibits differentiation and promotes insulin resistance

Serum amyloid A (SAA) is an acute-phase protein that has been recently correlated with obesity and insulin resistance. Therefore, we first examined whether human recombinant SAA (rSAA) could affect the proliferation, differentiation and metabolism of 3T3-L1 preadipocytes. Preadipocytes were treated...

Full description

Saved in:
Bibliographic Details
Published in:International Journal of Obesity 2012-08, Vol.36 (8), p.1032-1039
Main Authors: FILIPPIN-MONTEIRO, F. B, OLIVEIRA, Em De, SANDRI, S, KNEBEL, F. H, ALBUQUERQUE, R. C, CAMPA, A
Format: Article
Language:English
Subjects:
3T3-L1 Cells - metabolism
Adipocytes
Adipocytes - metabolism
Animals
Biological and medical sciences
Body fat
Cell cycle
Cell Differentiation - genetics
Cell growth
Cell Proliferation
Chronic illnesses
Cytokines
Deoxyglucose - metabolism
Development and progression
Diabetes
Fat cells
Flow Cytometry
Glucose
Glycerol
Growth factors
Humans
Insulin
Insulin resistance
Insulin Resistance - genetics
Intercellular Signaling Peptides and Proteins
Kinetics
Lipids
Medical sciences
Metabolic diseases
Metabolism
Mice
Mice, Obese
Morbidity
Obesity
Original
Pharmaceutical sciences
Physiological aspects
Proteins
Real-Time Polymerase Chain Reaction
Risk factors
RNA, Messenger - metabolism
Sensitivity
Serum Amyloid A Protein - genetics
Serum Amyloid A Protein - metabolism
Transcription factors
Tumor necrosis factor-TNF
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Serum amyloid A (SAA) is an acute-phase protein that has been recently correlated with obesity and insulin resistance. Therefore, we first examined whether human recombinant SAA (rSAA) could affect the proliferation, differentiation and metabolism of 3T3-L1 preadipocytes. Preadipocytes were treated with rSAA and analyzed for changes in viability and [³H-methyl]-thymidine incorporation as well as cell cycle perturbations using flow cytometry analysis. The mRNA expression profiles of adipogenic factors during the differentiation protocol were also analyzed using real-time PCR. After differentiation, 2-deoxy-[1,2-³H]-glucose uptake and glycerol release were evaluated. rSAA treatment caused a 2.6-fold increase in cell proliferation, which was consistent with the results from flow cytometry showing that rSAA treatment augmented the percentage of cells in the S phase (60.9±0.54%) compared with the control cells (39.8±2.2%, (***) P
ISSN:0307-0565
1476-5497
DOI:10.1038/ijo.2011.193