Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis

Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis

Myosin 1E (Myo1E) is recruited to sites of clathrin-mediated endocytosis coincident with a burst of actin assembly. The recruitment dynamics and lifetime of Myo1E are similar to those of tagged actin polymerization regulatory proteins. Like inhibition of actin assembly, depletion of Myo1E causes red...

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Bibliographic Details
Published in:Molecular biology of the cell 2012-08, Vol.23 (15), p.2891-2904
Main Authors: Cheng, Jackie, Grassart, Alexandre, Drubin, David G
Format: Article
Language:eng
Subjects:
3T3 Cells
Actin Cytoskeleton - metabolism
Actin-Related Protein 2-3 Complex - metabolism
Actins - metabolism
Animals
Cell Membrane
Cell Movement
Chlorocebus aethiops
Clathrin - metabolism
COS Cells
Endocytosis
HeLa Cells
Humans
Membrane Transport Proteins - metabolism
Mice
Myosin Type I
Myosins - metabolism
Protein Transport
Transferrin - metabolism
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Summary:Myosin 1E (Myo1E) is recruited to sites of clathrin-mediated endocytosis coincident with a burst of actin assembly. The recruitment dynamics and lifetime of Myo1E are similar to those of tagged actin polymerization regulatory proteins. Like inhibition of actin assembly, depletion of Myo1E causes reduced transferrin endocytosis and a significant delay in transferrin trafficking to perinuclear compartments, demonstrating an integral role for Myo1E in these actin-mediated steps. Mistargeting of GFP-Myo1E or its src-homology 3 domain to mitochondria results in appearance of WIP, WIRE, N-WASP, and actin filaments at the mitochondria, providing evidence for Myo1E's role in actin assembly regulation. These results suggest for mammalian cells, similar to budding yeast, interdependence in the recruitment of type I myosins, WIP/WIRE, and N-WASP to endocytic sites for Arp2/3 complex activation to assemble F-actin as endocytic vesicles are being formed.
ISSN:1059-1524
1939-4586