DNA sequence of the control region of phage D108: the N-terminal amino acid sequences of repressor and transposase are similar both in phage D108 and in its relative, phage Mu

We have determined the DNA sequence of the control region of phage D108 up to position 1419 at the left end of the phage genome. Open reading frames for the repressor gene, ner gene, and the 5' part of the A gene (which codes for transposase) are found in the sequence. The genetic organization...

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Bibliographic Details
Published in:Nucleic acids research 1986-05, Vol.14 (9), p.3813-3825
Main Authors: Mizuuchi, Michiyo, Weiberg, Robert A., Mizuuchi, Kiyoshi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological and medical sciences
Coliphages - genetics
DNA Transposable Elements
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Gene expression
genes
Genes, Regulator
Genes, Viral
Molecular and cellular biology
Molecular genetics
nucleotide sequence
Nucleotidyltransferases - genetics
phage D108
Phage Mu
Repressor Proteins - genetics
repressors
Sequence Homology, Nucleic Acid
Transcription Factors - genetics
Transposases
transposonase
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Summary:We have determined the DNA sequence of the control region of phage D108 up to position 1419 at the left end of the phage genome. Open reading frames for the repressor gene, ner gene, and the 5' part of the A gene (which codes for transposase) are found in the sequence. The genetic organization of this region of phage D108 is quite similar to that of phage Mu in spite of considerable divergence, both in the nucleotide sequence and in the amino acid sequences of the regulatory proteins of the two phages. The N-terminal amino acid sequences of the transposases of the two phages also share only limited homology. On the other hand, a significant amino acid sequence homology was found within each phage between the N-terminal parts of the repressor and transposase. We propose that the N-terminal domains of the repressor and transposase of each phage interact functionally in the process of making the decision between the lytic and the lysogenic mode of growth.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/14.9.3813