High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance

Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a p...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2012-07, Vol.109 (28), p.E1972-E1979
Main Authors: Bart, Rebecca, Cohn, Megan, Kassen, Andrew, McCallum, Emily J, Shybut, Mikel, Petriello, Annalise, Krasileva, Ksenia, Dahlbeck, Douglas, Medina, Cesar, Alicai, Titus, Kumar, Lava, Moreira, Leandro M, Neto, Júlio Rodrigues, Verdier, Valerie, Santana, María Angélica, Kositcharoenkul, Nuttima, Vanderschuren, Hervé, Gruissem, Wilhelm, Bernal, Adriana, Staskawicz, Brian J
Format: Article
Language:English
Subjects:
animals
Area Under Curve
Biological Sciences
blight
Cassava
developing countries
Disease Progression
Evolution
genome
Genome, Bacterial
Genomics
Geography
Gram-negative bacteria
Immunity, Innate
Life sciences
Manihot - metabolism
Manihot - microbiology
Manihot/metabolism/microbiology
Microbiologie
Microbiology
Models, Genetic
Molecular Sequence Data
people
Phylogenetics
Phylogeny
Plant diseases
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Diseases/genetics/microbiology
plant pathogens
PNAS Plus
Polymorphism, Genetic
Polymorphism, Single Nucleotide
proteins
Sciences du vivant
sequence analysis
Sequence Analysis, DNA - methods
Time Factors
virulence
Xanthomonas axonopodis
Xanthomonas axonopodis - metabolism
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Summary:Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies.
ISSN:0027-8424
1091-6490
1091-6490
DOI:10.1073/pnas.1208003109