Desulfuribacillus alkaliarsenatis gen. nov. sp. nov., a deep-lineage, obligately anaerobic, dissimilatory sulfur and arsenate-reducing, haloalkaliphilic representative of the order Bacillales from soda lakes

An anaerobic enrichment culture inoculated with a sample of sediments from soda lakes of the Kulunda Steppe with elemental sulfur as electron acceptor and formate as electron donor at pH 10 and moderate salinity inoculated with sediments from soda lakes in Kulunda Steppe (Altai, Russia) resulted in...

Full description

Saved in:

Bibliographic Details
Published in:	Extremophiles : life under extreme conditions 2012-07, Vol.16 (4), p.597-605
Main Authors:	Sorokin, D. Y., Tourova, T. P., Sukhacheva, M. V., Muyzer, G.
Format:	Article
Language:	eng
Subjects:	Aquatic ecosystems Arsenates - metabolism Bacillales - classification Bacillales - cytology Bacillales - genetics Bacillales - isolation & purification Bacillales - metabolism Base Sequence Biochemistry Biomedical and Life Sciences Biotechnology Hydrogen-Ion Concentration Lakes Lakes - microbiology Life Sciences Microbial Ecology Microbiology Molecular Sequence Data Original Paper Oxidation-Reduction RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Sediments Siberia Sulfur - metabolism Water Microbiology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	An anaerobic enrichment culture inoculated with a sample of sediments from soda lakes of the Kulunda Steppe with elemental sulfur as electron acceptor and formate as electron donor at pH 10 and moderate salinity inoculated with sediments from soda lakes in Kulunda Steppe (Altai, Russia) resulted in the domination of a Gram-positive, spore-forming bacterium strain AHT28. The isolate is an obligate anaerobe capable of respiratory growth using elemental sulfur, thiosulfate (incomplete reduction) and arsenate as electron acceptor with H 2 , formate, pyruvate and lactate as electron donor. Growth was possible within a pH range from 9 to 10.5 (optimum at pH 10) and a salt concentration at pH 10 from 0.2 to 2 M total Na + (optimum at 0.6 M). According to the phylogenetic analysis, strain AHT28 represents a deep independent lineage within the order Bacillales with a maximum of 90 % 16S rRNA gene similarity to its closest cultured representatives. On the basis of its distinct phenotype and phylogeny, the novel haloalkaliphilic anaerobe is suggested as a new genus and species, Desulfuribacillus alkaliarsenatis (type strain AHT28 T = DSM24608 T = UNIQEM U855 T ).
ISSN:	1431-0651 1433-4909