Nucleotide sequence of a cDNA encoding ribosomal acidic phosphoprotein P1 from Dictyostelium discoideum: identification of a novel carboxy-terminal sequence in 'A' proteins

We report the nucleotide sequence of a cDNA clone encoding the complete amino acid sequence of Dictyostelium discoideum ribosomal acidic protein P1. The cDNA was isolated from a lambda gt11 expression library, constructed in this vector by insertion of cDNAs obtained from Dictyostelium discoideum (s...

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Bibliographic Details
Published in:Nucleic acids research 1991-03, Vol.19 (6), p.1340-1340
Main Authors: Prieto, J, Candel, E, Coloma, A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
Biological and medical sciences
Dictyostelium
Dictyostelium - metabolism
Dictyostelium discoideum
DNA
DNA - genetics
DNA, Fungal - genetics
embl/x56193
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequences
phosphoproteins
Phosphoproteins - genetics
ribosomal acidic phosphoprotein P1
Ribosomal Proteins - genetics
ribosomes
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Summary:We report the nucleotide sequence of a cDNA clone encoding the complete amino acid sequence of Dictyostelium discoideum ribosomal acidic protein P1. The cDNA was isolated from a lambda gt11 expression library, constructed in this vector by insertion of cDNAs obtained from Dictyostelium discoideum (strain AX-3) 4 hours-starved cells. The screening of this library was performed using polyclonal antibodies, raised against Dictyostelium ribosomal proteins P1 and P2 which can be selectively released from 80S ribosomal particles with salt/ethanol washes. The insert found in a lambda positive clone was subcloned into pUC plasmid and both strands were sequenced by the chain termination method. The cDNA was 442 nucleotides in length and contained the translation start and stop codons. It also has a 5' noncoding sequence of 12 bases, an open reading frame of 339, and a 3' noncoding sequence of 91 which includes a polyadenylation recognition sequence- AATAAA-located at position 418-423, as well as a poly(A) stretch. The open reading frame encoded a protein of 113 amino acid residues. The primary structure inferred from the cDNA sequence corresponds to a negatively charged protein that includes sequential stretches of hydrophobic and hydrophilic residues.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.6.1340