A catalytic 13-mer ribozyme

A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten...

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Bibliographic Details
Published in:Nucleic acids research 1989-02, Vol.17 (4), p.1371-1377
Main Authors: Jeffries, A C, Symons, R H
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Fishes
Kinetics
Molecular Sequence Data
Nucleic Acid Conformation
Oligodeoxyribonucleotides
RNA Precursors - metabolism
RNA Splicing
RNA, Catalytic
RNA, Ribosomal - metabolism
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Summary:A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten kinetic parameters for the reaction were; Km 1.3 microM, Vmax 0.012 microM min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.4.1371