CD8+ T Cells Provide an Immunologic Signature of Tuberculosis in Young Children

The immunologic events surrounding primary Mycobacterium tuberculosis infection and development of tuberculosis remain controversial. Young children who develop tuberculosis do so quickly after first exposure, thus permitting study of immune response to primary infection and disease. We hypothesized...

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Published in:American journal of respiratory and critical care medicine 2012-01, Vol.185 (2), p.206-212
Main Authors: LANCIONI, Christina, NYENDAK, Melissa, BYRD, Laura, SWARBRICK, Gwendolyn, SCOTT, Christine, JOHNSON, Denise F, MALONE, Lashaunda, MUDIDO-MUSOKE, Philipa, BOOM, W. Henry, LEWINSOHN, David M, LEWINSOHN, Deborah A, KIGULI, Sarah, ZALWANGO, Sarah, MORI, Tomi, MAYANJA-KIZZA, Harriet, BALYEJUSA, Stephen, NULL, Megan, BASEKE, Joy, MULINDWA, Deo
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antigens, Bacterial - immunology
Bacterial Proteins - immunology
Biological and medical sciences
Biomarkers - blood
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
CD8-Positive T-Lymphocytes - immunology
Child
Child, Preschool
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Female
Hospitalization
Humans
Intensive care medicine
Interferon-gamma - blood
Male
Medical sciences
Mycobacterium tuberculosis - immunology
Recombinant Fusion Proteins - immunology
Statistics, Nonparametric
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Tuberculosis, Pulmonary - immunology
Uganda
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Summary:The immunologic events surrounding primary Mycobacterium tuberculosis infection and development of tuberculosis remain controversial. Young children who develop tuberculosis do so quickly after first exposure, thus permitting study of immune response to primary infection and disease. We hypothesized that M. tuberculosis-specific CD8(+) T cells are generated in response to high bacillary loads occurring during tuberculosis. To determine if M. tuberculosis-specific T cells are generated among healthy children exposed to M. tuberculosis and children with tuberculosis. Enzyme-linked immunosorbent spot assays were used to measure IFN-γ production in response to M. tuberculosis-specific proteins ESAT-6/CFP-10 by peripheral blood mononuclear cells and CD8(+) T cells isolated from Ugandan children hospitalized with tuberculosis (n = 96) or healthy tuberculosis contacts (n = 62). The proportion of positive CD8(+) T-cell assays and magnitude of CD8(+) T-cell responses were significantly greater among young (
ISSN:1073-449X
1535-4970
DOI:10.1164/rccm.201107-1355OC