Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance

The oxidative burst is an early response to pathogen attack leading to the production of reactive oxygen species (ROS) including hydrogen peroxide. Two major mechanisms involving either NADPH oxidases or peroxidases that may exist singly or in combination in different plant species have been propose...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2006-09, Vol.47 (6), p.851-863
Main Authors: Bindschedler, Laurence V, Dewdney, Julia, Blee, Kris A, Stone, Julie M, Asai, Tsuneaki, Plotnikov, Julia, Denoux, Carine, Hayes, Tezni, Gerrish, Chris, Davies, Dewi R, Ausubel, Frederick M, Bolwell, G. Paul
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
apoplastic peroxidase
Arabidopsis
Arabidopsis - enzymology
Arabidopsis - microbiology
Arabidopsis - physiology
Arabidopsis thaliana
Biological and medical sciences
Botany
Fumonisins - metabolism
Fundamental and applied biological sciences. Psychology
Fungi - pathogenicity
Fusarium oxysporum
Gene Expression Profiling
Genetics and breeding of economic plants
Oxidation
oxidative burst
Pathogens
Peroxidases - metabolism
Pest resistance
Plant mitochondria
Plant pathogens
Plants, Genetically Modified
Quantitative genetics
Reactive Oxygen Species
Respiratory Burst
Reverse Transcriptase Polymerase Chain Reaction
Transgenic plants
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:The oxidative burst is an early response to pathogen attack leading to the production of reactive oxygen species (ROS) including hydrogen peroxide. Two major mechanisms involving either NADPH oxidases or peroxidases that may exist singly or in combination in different plant species have been proposed for the generation of ROS. We identified an Arabidopsis thaliana azide-sensitive but diphenylene iodonium-insensitive apoplastic oxidative burst that generates H₂O₂ in response to a Fusarium oxysporum cell-wall preparation. Transgenic Arabidopsis plants expressing an anti-sense cDNA encoding a type III peroxidase, French bean peroxidase type 1 (FBP1) exhibited an impaired oxidative burst and were more susceptible than wild-type plants to both fungal and bacterial pathogens. Transcriptional profiling and RT-PCR analysis showed that the anti-sense (FBP1) transgenic plants had reduced levels of specific peroxidase-encoding mRNAs, including mRNAs corresponding to Arabidopsis genes At3g49120 (AtPCb) and At3g49110 (AtPCa) that encode two class III peroxidases with a high degree of homology to FBP1. These data indicate that peroxidases play a significant role in generating H₂O₂ during the Arabidopsis defense response and in conferring resistance to a wide range of pathogens.
ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313x.2006.02837.x