Coordinated regulation of caveolin-1 and Rab11a in apical recycling compartments of polarized epithelial cells

Recent studies have identified caveolin-1, a protein best known for its functions in caveolae, in apical endocytic recycling compartments in polarized epithelial cells. However, very little is known about the regulation of caveolin-1 in the endocytic recycling pathway. To address this question, in t...

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Bibliographic Details
Published in:Experimental cell research 2012-01, Vol.318 (2), p.103-113
Main Authors: Lapierre, Lynne A., Ducharme, Nicole A., Drake, Kimberly R., Goldenring, James R., Kenworthy, Anne K.
Format: Article
Language:English
Subjects:
Animals
Apical recycling
Caveolin 1 - metabolism
Caveolin-1
Cell Line
Cell Polarity
Cellular biology
Dogs
Endocytosis
Endosomes
Epithelial Cells - metabolism
Indexing in process
MDCK
Microtubules - metabolism
Polarized epithelial cells
Proteins
rab GTP-Binding Proteins - metabolism
Rab11-FIP2
Rab11a
Signal transduction
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Summary:Recent studies have identified caveolin-1, a protein best known for its functions in caveolae, in apical endocytic recycling compartments in polarized epithelial cells. However, very little is known about the regulation of caveolin-1 in the endocytic recycling pathway. To address this question, in the current study we compared the relationship between compartments enriched in sub-apical caveolin-1 and Rab11a, a well-defined marker of apical recycling endosomes, using polarized MDCK cells as a model. We show that caveolin-1-containing vesicles define a compartment that partially overlaps with Rab11a, and that the distribution of subapical caveolin-1 and Rab11a shows a similar dependence on microtubule disruption. Mutants of the Rab11a effector, Rab11-FIP2 also altered the localization of caveolin-1. These findings indicate that caveolin-1 is coordinately regulated with Rab11a within the apical recycling system of polarized epithelial cells, suggesting that the two proteins are components of the same pathway.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2011.10.010