Long noncoding RNAs are generated from the mitochondrial genome and regulated by nuclear-encoded proteins

Human mitochondrial long noncoding RNAs (lncRNAs) have not been described to date. By analysis of deep-sequencing data we have identified three lncRNAs generated from the mitochondrial genome and confirmed their expression by Northern blotting and strand-specific qRT-PCR. We show that the abundance...

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Bibliographic Details
Published in:RNA (Cambridge) 2011-12, Vol.17 (12), p.2085-2093
Main Authors: Rackham, Oliver, Shearwood, Anne-Marie J, Mercer, Tim R, Davies, Stefan M K, Mattick, John S, Filipovska, Aleksandra
Format: Article
Language:English
Subjects:
Base Sequence
Cell Nucleus - genetics
Gene Expression Regulation
Genome, Mitochondrial
HeLa Cells
Humans
Mitochondria - genetics
Mitochondria - metabolism
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Molecular Sequence Data
Nucleic Acid Conformation
Organ Specificity - genetics
Ribonuclease P - genetics
Ribonuclease P - metabolism
RNA Stability
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
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Summary:Human mitochondrial long noncoding RNAs (lncRNAs) have not been described to date. By analysis of deep-sequencing data we have identified three lncRNAs generated from the mitochondrial genome and confirmed their expression by Northern blotting and strand-specific qRT-PCR. We show that the abundance of these lncRNAs is comparable to their complementary mRNAs and that nuclear-encoded mitochondrial proteins involved in RNA processing regulate their expression. We also identify the 5' and 3' transcript ends of the three lncRNAs and show that mitochondrial RNase P protein 1 (MRPP1) is important for the processing of these transcripts. Finally, we show that mitochondrial lncRNAs form intermolecular duplexes and that their abundance is cell- and tissue-specific, suggesting a functional role in the regulation of mitochondrial gene expression.
ISSN:1355-8382
1469-9001
DOI:10.1261/rna.029405.111