The use of BMP-2 coupled – Nanosilver-PLGA composite grafts to induce bone repair in grossly infected segmental defects

Abstract Healing of contaminated/infected bone defects is a significant clinical challenge. Prevalence of multi-antibiotic resistant organisms has renewed interest in the use of antiseptic silver as an effective, but less toxic antimicrobial with decreased potential for bacterial resistance. In this...

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Bibliographic Details
Published in:Biomaterials 2010-12, Vol.31 (35), p.9293-9300
Main Authors: Zheng, Zhong, Yin, Wei, Zara, Janette N, Li, Weiming, Kwak, Jinny, Mamidi, Rachna, Lee, Min, Siu, Ronald K, Ngo, Richard, Wang, Joyce, Carpenter, Doug, Zhang, Xinli, Wu, Benjamin, Ting, Kang, Soo, Chia
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Anti-Infective Agents - chemistry
Anti-Infective Agents - therapeutic use
Antimicrobial
Bone morphogenetic protein 2 (BMP-2)
Bone Morphogenetic Protein 2 - adverse effects
Bone Morphogenetic Protein 2 - chemistry
Bone Morphogenetic Protein 2 - therapeutic use
Bone Regeneration - drug effects
Bone repair
Cell Line
Cell Proliferation - drug effects
Dentistry
Immunohistochemistry
Lactic Acid - adverse effects
Lactic Acid - chemistry
Lactic Acid - therapeutic use
Male
Mice
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Nanocomposites - adverse effects
Nanocomposites - chemistry
Nanocomposites - therapeutic use
Nanocomposites - ultrastructure
Nanosilver particle
Nanotechnology
Polyglycolic Acid - adverse effects
Polyglycolic Acid - chemistry
Polyglycolic Acid - therapeutic use
Prostheses and Implants - adverse effects
Prostheses and Implants - ultrastructure
Rats
Rats, Sprague-Dawley
Silver - chemistry
Silver - therapeutic use
Wound Healing - drug effects
X-Ray Microtomography
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Summary:Abstract Healing of contaminated/infected bone defects is a significant clinical challenge. Prevalence of multi-antibiotic resistant organisms has renewed interest in the use of antiseptic silver as an effective, but less toxic antimicrobial with decreased potential for bacterial resistance. In this study, we demonstrated that metallic nanosilver particles (with a size of 20–40 nm)-poly(lactic- co -glycolic acid) (PLGA) composite grafts have strong antibacterial properties. In addition, nanosilver particles-PLGA composite grafts did not inhibit adherence, proliferation, alkaline phosphatase activity, or mineralization of ongrowth MC3T3-E1 pre-osteoblasts compared to PLGA controls. Furthermore, nanosilver particles did not affect the osteoinductivity of bone morphogenetic protein 2 (BMP-2). Infected femoral defects implanted with BMP-2 coupled 2.0% nanosilver particles-PLGA composite grafts healed in 12 weeks without evidence of residual bacteria. In contrast, BMP-2 coupled PLGA control grafts failed to heal in the presence of continued bacterial colonies. Our results indicate that nanosilver of defined particle size is bactericidal without discernable in vitro and in vivo cytotoxicity or negative effects on BMP-2 osteoinductivity, making it an ideal antimicrobial for bone regeneration in infected wounds.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2010.08.041