Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601

The virulence-attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China. We studied the genetic variations of IPAV that render it avirulent via comparative analysis against the pathogenic L. interro...

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Bibliographic Details
Published in:Cell research 2011-08, Vol.21 (8), p.1210-1229
Main Authors: Zhong, Yi, Chang, Xiao, Cao, Xing-Jun, Zhang, Yan, Zheng, Huajun, Zhu, Yongzhang, Cai, Chengsong, Cui, Zelin, Zhang, Yunyi, Li, Yuan-Yuan, Jiang, Xiu-Gao, Zhao, Guo-Ping, Wang, Shengyue, Li, Yixue, Zeng, Rong, Li, Xuan, Guo, Xiao-Kui
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
Chromosomes, Bacterial
Genes, Bacterial
Genetic Variation
GTP结合蛋白
Guinea Pigs
Leptospira interrogans
Leptospira interrogans - genetics
Leptospira interrogans - metabolism
Leptospira interrogans - pathogenicity
Models, Animal
Molecular Sequence Data
Mutation
Original
Proteome - analysis
Sequence Alignment
Up-Regulation
Virulence - genetics
丝氨酸/苏氨酸激酶
全基因组
应变
毒力
比较蛋白质组学
谷氨酰胺合成酶
钩端螺旋体
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Summary:The virulence-attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China. We studied the genetic variations of IPAV that render it avirulent via comparative analysis against the pathogenic L. interrogans serovar Lai strain 56601. The complete genome sequence of the IPAV strain was determined and used to compare with, and then rectify and reannotate the genome sequence of strain 56601. Aside from their highly similar genomic structure and gene order, a total of 33 insertions, 53 deletions and 301 single-nucleotide variations （SNVs） were detected throughout the genome of IPAV directly affecting 101 genes, either in their 5＇ upstream region or within their coding region. Among them, the majority of the 44 functional genes are involved in signal transduction, stress response, transmembrane transport and nitrogen metabolism. Comparative proteomic analysis based on quantitative liquid chromatography （LC）-MS/MS data revealed that among 1 627 selected pairs of orthologs, 174 genes in the IPAV strain were upregulated, with enrichment mainly in classes of energy production and lipid metabolism. In contrast, 228 genes in strain 56601 were upregulated, with the majority enriched in the categories of protein translation and DNA replication/repair. The combination of genomic and proteomic approaches illustrated that altered expression or mutations in critical genes, such as those encoding a Ser/Thr kinase, carbon-starvation protein CstA, glutamine synthetase, GTP-binding protein BipA, ribonucleotide- diphosphate reductase and phosphate transporter, and alterations in the translational profile of lipoproteins or outer membrane proteins are likely to account for the virulence attenuation in strain IPAV.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2011.46