Mapping of the genes coding for the two major vaccinia virus core polypeptides

We have mapped the genes coding for two major structural polypeptides of the vaccinia virus core by hybrid selection and transcriptional mapping. First, RNA was selected by hybridization to restriction fragments of the vaccinia virus genome, translated in vitro and the products were immunoprecipitat...

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Bibliographic Details
Published in:Nucleic acids research 1984-06, Vol.12 (12), p.4835-4848
Main Authors: Wittek, Riccardo, Richner, Barbara, Hiller, Gerhard
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
DNA Restriction Enzymes
Genes
Genes, Viral
Kidney
Molecular Weight
Nucleic Acid Hybridization
Rabbits
RNA, Viral - genetics
Transcription, Genetic
Vaccinia virus
Vaccinia virus - genetics
Viral Proteins - genetics
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Summary:We have mapped the genes coding for two major structural polypeptides of the vaccinia virus core by hybrid selection and transcriptional mapping. First, RNA was selected by hybridization to restriction fragments of the vaccinia virus genome, translated in vitro and the products were immunoprecipitated with antibodies against the two polypeptides. This approach allowed us to map the genes to the left hand end of the largest Hind III restriction fragment of 50 kilobase pairs. Second, transcriptional mapping of this region of the genome revealed the presence of the two expected RNAs. Both RNAs are transcribed from the leftward reading strand and the 5′-ends of the genes are separated by about 7.5 kilobase pairs of DNA. Thus, two genes encoding structural polypeptides with a similar location in the vaccinia virus particle are clustered at approximately 105 kilobase pairs from the left hand end of the 180 kilobase pair vaccinia virus genome.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/12.12.4835