Transcription of the chicken myb proto-oncogene starts within a CpG island

The nucleotide sequence of an 8.2-kb DNA fragment from the 5' proximal part of the chicken myb proto-oncogene spanning 1761 nucleotides upstream and 6436 nucleotides downstream from a presumed c-myb initiation codon was determined. A 3.3-kb G + C-rich region found in this sequence had also othe...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1989-07, Vol.17 (14), p.5651-5664
Main Authors: DVORAK, M, URBANEK, P, BARTUNEK, P, PACES, V, VLACH, J, PECENKA, V, ARNOLD, L, TRAVNICEK, M, RIMAN, J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Chickens
Cloning, Molecular
Dinucleoside Phosphates - analysis
Endonucleases
Erythrocytes - analysis
Fundamental and applied biological sciences. Psychology
Male
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Organ Specificity
Promoter Regions, Genetic
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-myb
Proto-Oncogenes
Restriction Mapping
RNA Caps - genetics
Sequence Homology, Nucleic Acid
Single-Strand Specific DNA and RNA Endonucleases
Thymus Gland - analysis
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The nucleotide sequence of an 8.2-kb DNA fragment from the 5' proximal part of the chicken myb proto-oncogene spanning 1761 nucleotides upstream and 6436 nucleotides downstream from a presumed c-myb initiation codon was determined. A 3.3-kb G + C-rich region found in this sequence had also other features characterizing CpG islands, i.e. no CpG underrepresentation and lack of CpG methylation. In haematopoietic tissues c-myb mRNA synthesis starts in two major regions of the CpG island, namely 98 to 108 and 143 to 145 nucleotides upstream from the c-myb initiation codon. These two regions are in or close to the 124-bp evolutionarily conserved element located in the middle part of the CpG island. No alternative splicing of the 5' end of c-myb mRNA suggested earlier (1,2) was observed. The c-myb promoter contains neither TATA nor CAAT box-like structures at the usual positions. Instead, numerous potential Sp1 factor binding sites were found both upstream and downstream from the transcription initiation sites. Moreover, consensus v-myb protein DNA-binding sites were revealed in the promoter region and in sequences downstream from it.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.14.5651