Age-dependent expression of forkhead box O proteins in the duodenum of rats

The O subfamily of forkhead box（FoxO） proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B（IGF-1/PI3K/PKB） signal pathway.The objective of the present study was to examine the expressions of three members of FoxO proteins,FoxO1,FoxO3a,and...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2011-09, Vol.12 (9), p.730-735
Main Authors: Huang, Pan, Zhou, Zhen-qi, Huang, Rui-hua, Zhou, Bo, Wei, Quan-wei, Shi, Fang-xiong
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biomedical and Life Sciences
Biomedicine
Cytoplasm - metabolism
Duodenum - metabolism
Duodenum - pathology
Forkhead Box Protein O3
Forkhead Transcription Factors - metabolism
Immunohistochemistry - methods
Male
Mucous Membrane - metabolism
Nerve Tissue Proteins - metabolism
Phosphorylation
Rats
Rats, Sprague-Dawley
Time Factors
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Summary:The O subfamily of forkhead box（FoxO） proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B（IGF-1/PI3K/PKB） signal pathway.The objective of the present study was to examine the expressions of three members of FoxO proteins,FoxO1,FoxO3a,and FoxO4 in the duodenum of Sprague-Dawley rats at different ages.The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner.At Day 21,there were no detectable localization and expression of FoxO4 in the duodenum,while,at Months 2 and 6,localization and expression of FoxO4 were distinct.In addition,FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats,but was not detectable in the same area in 6-month-old rats.Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2-and 6-month marks,it was still not detectable at Day 21.Besides,FoxO1 was not detectable in all parts and stages.Taken together,our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1000298