RhoA and RhoC have distinct roles in migration and invasion by acting through different targets

Several studies suggest that RhoA and RhoC, despite their sequence similarity, have different roles in cell migration and invasion, but the molecular basis for this is not known. Using RNAi, we show that RhoA-depleted cells became elongated and extended multiple Rac1-driven narrow protrusions in 2D...

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Bibliographic Details
Published in:The Journal of cell biology 2011-05, Vol.193 (4), p.655-665
Main Authors: Vega, Francisco M, Fruhwirth, Gilbert, Ng, Tony, Ridley, Anne J
Format: Article
Language:English
Subjects:
Breast Neoplasms - enzymology
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cell adhesion & migration
Cell Line, Tumor
Cell Movement
Cell Polarity
Cell Shape
Cells
Female
Genotype
Genotype & phenotype
Humans
Kinases
Male
Molecules
Neoplasm Invasiveness
Phenotype
Prostatic Neoplasms - enzymology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
Proteins - metabolism
Pseudopodia - metabolism
rac1 GTP-Binding Protein - metabolism
rho GTP-Binding Proteins - genetics
rho GTP-Binding Proteins - metabolism
rho-Associated Kinases - metabolism
rhoA GTP-Binding Protein - genetics
rhoA GTP-Binding Protein - metabolism
rhoC GTP-Binding Protein
RNA Interference
Signal Transduction
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Summary:Several studies suggest that RhoA and RhoC, despite their sequence similarity, have different roles in cell migration and invasion, but the molecular basis for this is not known. Using RNAi, we show that RhoA-depleted cells became elongated and extended multiple Rac1-driven narrow protrusions in 2D and 3D environments, leading to increased invasion. These phenotypes were caused by combined but distinct effects of the Rho-regulated kinases ROCK1 and ROCK2. Depletion of ROCK2 induced multiple delocalized protrusions and reduced migratory polarity, whereas ROCK1 depletion selectively led to cell elongation and defective tail retraction. In contrast, RhoC depletion increased cell spreading and induced Rac1 activation around the periphery in broad lamellipodia, thereby inhibiting directed migration and invasion. These effects of RhoC depletion are mediated by the formin FMNL3, which we identify as a new target of RhoC but not RhoA. We propose that RhoA contributes to migratory cell polarity through ROCK2-mediated suppression of Rac1 activity in lamellipodia, whereas RhoC promotes polarized migration through FMNL3 by restricting lamellipodial broadening.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.201011038