The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain

Mediator was resolved from yeast as a multiprotein complex on the basis of its requirement for transcriptional activation in a fully defined system. Three groups of mediator polypeptides could be distinguished: the products of five SRB genes, identified as suppressors of carboxy-terminal domain (CTD...

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Bibliographic Details
Published in:Genes & development 1998-01, Vol.12 (1), p.45-54
Main Authors: Myers, L C, Gustafsson, C M, Bushnell, D A, Lui, M, Erdjument-Bromage, H, Tempst, P, Kornberg, R D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Binding Sites
DNA, Complementary
Fungal Proteins - genetics
Fungal Proteins - metabolism
Humans
Macromolecular Substances
Mediator Complex
Molecular Sequence Data
Multiprotein Complexes
Research Paper
RNA Polymerase II - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Mediator was resolved from yeast as a multiprotein complex on the basis of its requirement for transcriptional activation in a fully defined system. Three groups of mediator polypeptides could be distinguished: the products of five SRB genes, identified as suppressors of carboxy-terminal domain (CTD)-truncation mutants; products of four genes identified as global repressors; and six members of a new protein family, termed Med, thought to be primarily responsible for transcriptional activation. Notably absent from the purified mediator were Srbs 8, 9, 10, and 11, as well as members of the SWI/SNF complex. The CTD was required for function of mediator in vitro, in keeping with previous indications of involvement of the CTD in transcriptional activation in vivo. Evidence for human homologs of several mediator proteins, including Med7, points to similar mechanisms in higher cells.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.12.1.45