Simultaneous in vivo imaging of melanin and lipofuscin in the retina with photoacoustic ophthalmoscopy and autofluorescence imaging

We combined photoacoustic ophthalmoscopy (PAOM) with autofluorescence imaging for simultaneous in vivo imaging of dual molecular contrasts in the retina using a single light source. The dual molecular contrasts come from melanin and lipofuscin in the retinal pigment epithelium (RPE). Melanin and lip...

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Bibliographic Details
Published in:Journal of biomedical optics 2011-08, Vol.16 (8), p.080504-080504
Main Authors: Zhang, Xiangyang, Zhang, Hao F, Puliafito, Carmen A, Jiao, Shuliang
Format: Article
Language:English
Subjects:
Aging
Animals
Biocompatibility
Biomedical materials
Humans
Imaging
In vivo testing
In vivo tests
JBO Letters
Lipofuscin - analysis
Lipofuscin - chemistry
Melanin
Melanins - analysis
Melanins - chemistry
Ophthalmoscopy - methods
Photoacoustic Techniques - methods
Rats
Retina
Retinal Pigment Epithelium - chemistry
Spectrometry, Fluorescence
Surgical implants
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Summary:We combined photoacoustic ophthalmoscopy (PAOM) with autofluorescence imaging for simultaneous in vivo imaging of dual molecular contrasts in the retina using a single light source. The dual molecular contrasts come from melanin and lipofuscin in the retinal pigment epithelium (RPE). Melanin and lipofuscin are two types of pigments and are believed to play opposite roles (protective versus exacerbate) in the RPE in the aging process. We have successfully imaged the retina of pigmented and albino rats at different ages. The experimental results showed that multimodal PAOM system can be a potentially powerful tool in the study of age-related degenerative retinal diseases.
ISSN:1083-3668
1560-2281
1560-2281
DOI:10.1117/1.3606569