Cavernous Nerve Repair With Allogenic Adipose Matrix and Autologous Adipose-derived Stem Cells

Objectives To investigate whether adipose-derived matrix seeded with adipose-derived stem cells (ADSC) can facilitate the repair of injured cavernous nerves (CNs). Methods Human and rat adipose tissues were decellularized and fabricated into various forms, including adipose tissue-derived acellular...

Full description

Saved in:
Bibliographic Details
Published in:Urology (Ridgewood, N.J.) N.J.), 2011-06, Vol.77 (6), p.1509.e1-1509.e8
Main Authors: Lin, Guiting, Albersen, Maarten, Harraz, Ahmed M, Fandel, Thomas M, Garcia, Maurice, McGrath, Mary H, Konety, Badrinath R, Lue, Tom F, Lin, Ching-Shwun
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
Axons - pathology
Cell Transplantation
Erectile Dysfunction - pathology
Humans
Immunohistochemistry - methods
Inflammation
Male
Microscopy, Fluorescence - methods
Nerve Regeneration - physiology
Penis - innervation
Penis - surgery
Rats
Rats, Sprague-Dawley
Stem Cells - metabolism
Urology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives To investigate whether adipose-derived matrix seeded with adipose-derived stem cells (ADSC) can facilitate the repair of injured cavernous nerves (CNs). Methods Human and rat adipose tissues were decellularized and fabricated into various forms, including adipose tissue-derived acellular matrix thread (ADMT). ADMT seeded with ADSC were transplanted into subcutaneous space and examined for signs of inflammation. ADSC-seeded ADMTs were then used to repair CN injury in rats, followed by assessment of histology and erectile function. Results Adipose tissue can be fabricated into acellular matrices of various shapes and sizes, including threads and sheets. Seeding of ADMT occurred rapidly: within 24 hours, 55% of the surface was covered with ADSC and within 1 week, 90% was covered. Transplantation of the seeded ADMT into the subcutaneous space of an allogenic host showed no signs of inflammatory reaction. At 3 months after grafting into CN injury rats, approximately twice as many cells were found on seeded ADMT as on unseeded ADMT. The seeded ADMT also had various degrees of S100 and neuronal nitric oxide synthase expression, suggesting CN axonal ingrowth. Rats grafted with seeded ADMT overall had the best erectile function recovery when compared with those grafted with unseeded ADMT and those ungrafted. However, as a result of large variations, the differences did not reach statistic significance ( P = .07). Conclusions Grafting of ADSC-seeded matrix resulted in a substantial recovery of erectile function and improvement of histology. However, further refinement of the matrix architecture is needed to improve the success rate.
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2010.12.076