Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity

Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based o...

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Bibliographic Details
Published in:Biotechnology research international 2011, Vol.2011 (2011), p.1-4
Main Authors: Natalia, Dessy, Kohlmann, Christina, Ansorge-Schumacher, Marion B., Greiner, Lasse
Format: Article
Language:English
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Summary:Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.
ISSN:2090-3138
2090-3146
DOI:10.4061/2011/478925