Exploring the neutral invertase–oxidative stress defence connection in Arabidopsis thaliana

Over the past decades, considerable advances have been made in understanding the crucial role and the regulation of sucrose metabolism in plants. Among the various sucrose-catabolizing enzymes, alkaline/neutral invertases (A/NInvs) have long remained poorly studied. However, recent findings have dem...

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Bibliographic Details
Published in:Journal of experimental botany 2011-07, Vol.62 (11), p.3849-3862
Main Authors: Xiang, Li, Le Roy, Katrien, Bolouri-Moghaddam, Mohammad-Reza, Vanhaecke, Mieke, Lammens, Willem, Rolland, Filip, Van den Ende, Wim
Format: Article
Language:English
Subjects:
Abscisic Acid - metabolism
Antioxidants
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis - physiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Ascorbate Peroxidases
beta-Fructofuranosidase - genetics
beta-Fructofuranosidase - metabolism
Biological and medical sciences
Cytosol - enzymology
Enzymes
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Plant
Hydrogen Peroxide - metabolism
Mitochondria
Mitochondria - enzymology
Mitochondria - genetics
Oxidative Stress
Peroxidases - genetics
Peroxidases - metabolism
Plant cells
Plant Growth Regulators - metabolism
Plant Leaves - genetics
Plant Leaves - metabolism
Plant Leaves - physiology
Plant physiology
Plant physiology and development
Plants
Protoplasts
Protoplasts - enzymology
Protoplasts - metabolism
Reactive oxygen species
Reactive Oxygen Species - metabolism
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Summary:Over the past decades, considerable advances have been made in understanding the crucial role and the regulation of sucrose metabolism in plants. Among the various sucrose-catabolizing enzymes, alkaline/neutral invertases (A/NInvs) have long remained poorly studied. However, recent findings have demonstrated the presence of A/N-Invs in various organelles in addition to the cytosol, and their importance for plant development and stress tolerance. A cytosolic (At-A/N-InvG, At1g35580) and a mitochondrial (At-A/N-InvA, At1g56560) member of the A/N-Invs have been analysed in more detail in Arabidopsis and it was found that At-A/N-InvA knockout plants show an even more severe growth phenotype than At-A/N-InvG knockout plants. The absence of either A/N-Inv was associated with higher oxidative stress defence gene expression, while transient overexpression of At-A/N-InvA and At-A/N-InvG in leaf mesophyll protoplasts down-regulated the oxidative stress-responsive ascorbate peroxidase 2 (APX2) promoter. Moreover, up-regulation of the APX2 promoter by hydrogen peroxide or abscisic acid could be blocked by adding metabolizable sugars or ascorbate. A hypothetical model is proposed in which both mitochondrial and cytosolic A/N-Invs can generate glucose as a substrate for mitochondria-associated hexokinase, contributing to mitochondrial reactive oxygen species homeostasis.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/err069