Cell-autonomous regulation of hematopoietic stem cell cycling activity by ATP

Extracellular nucleotides regulate many cellular functions through activation of purinergic receptors in the plasma membrane. Here, we show that in hematopoietic stem cell (HSC), ATP is stored in vesicles and released in a calcium-sensitive manner. HSC expresses ATP responsive P2X receptors and in v...

Full description

Saved in:
Bibliographic Details
Published in:Cell death and differentiation 2011-03, Vol.18 (3), p.396-404
Main Authors: Casati, A, Frascoli, M, Traggiai, E, Proietti, M, Schenk, U, Grassi, F
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Adenosine Triphosphate - pharmacology
Adenosine Triphosphate - secretion
Animals
Cell cycle
Cell Cycle - drug effects
Cell death
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Chronic Disease
Cytoplasmic Vesicles - drug effects
Cytoplasmic Vesicles - secretion
Granulocytes
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Hematopoietic Stem Cells - secretion
Inflammation - pathology
Interferon
Mice
Myeloid Cells - cytology
Myeloid Cells - drug effects
Myeloid Cells - metabolism
Original Paper
Physiology
Purinergic P2X Receptor Antagonists - pharmacology
Signal transduction
Signal Transduction - drug effects
Stem cells
Toll-Like Receptors - metabolism
Tumor necrosis factor-TNF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extracellular nucleotides regulate many cellular functions through activation of purinergic receptors in the plasma membrane. Here, we show that in hematopoietic stem cell (HSC), ATP is stored in vesicles and released in a calcium-sensitive manner. HSC expresses ATP responsive P2X receptors and in vitro pharmacological P2X antagonism restrained hematopoietic progenitors proliferation, but not myeloid differentiation. In mice suffering from chronic inflammation, HSCs were significantly expanded and their cycling activity was sensitive to treatment with the P2X antagonist periodate-oxidized 2,3-dialdehyde ATP. Our results indicate that ATP acts as an autocrine stimulus in regulating HSCs pool size.
ISSN:1350-9047
1476-5403
DOI:10.1038/cdd.2010.107