Screening for resistance against Pseudomonas syringae in rice-FOX Arabidopsis lines identified a putative receptor-like cytoplasmic kinase gene that confers resistance to major bacterial and fungal pathogens in Arabidopsis and rice

Summary Approximately 20 000 of the rice‐FOX Arabidopsis transgenic lines, which overexpress 13 000 rice full‐length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overe...

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Published in:Plant biotechnology journal 2011-05, Vol.9 (4), p.466-485
Main Authors: Dubouzet, Joseph G., Maeda, Satoru, Sugano, Shoji, Ohtake, Miki, Hayashi, Nagao, Ichikawa, Takanari, Kondou, Youichi, Kuroda, Hirofumi, Horii, Yoko, Matsui, Minami, Oda, Kenji, Hirochika, Hirohiko, Takatsuji, Hiroshi, Mori, Masaki
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - microbiology
Biological and medical sciences
Biotechnology
Cloning, Molecular
Colletotrichum - pathogenicity
disease resistance
FOX hunting system
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Genetic Variation
Immunity, Innate
Magnaporthe - pathogenicity
Original
Oryza - enzymology
Oryza - genetics
Oryza - microbiology
Plant Diseases - genetics
Plant Diseases - microbiology
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Plants, Genetically Modified - microbiology
Pseudomonas syringae
Pseudomonas syringae - pathogenicity
receptor-like cytoplasmic kinase
rice
Transgenes
Xanthomonas - pathogenicity
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Summary:Summary Approximately 20 000 of the rice‐FOX Arabidopsis transgenic lines, which overexpress 13 000 rice full‐length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determined in 72 lines that showed consistent resistance after three independent screens. Pst DC3000 resistance was verified for 19 genes by characterizing other independent Arabidopsis lines for the same genes in the original rice‐FOX hunting population or obtained by reintroducing the genes into ecotype Columbia by floral dip transformation. Thirteen lines of these 72 selections were also resistant to the fungal pathogen Colletotrichum higginsianum. Eight genes that conferred resistance to Pst DC3000 in Arabidopsis have been introduced into rice for overexpression, and transformants were evaluated for resistance to the rice bacterial pathogen, Xanthomonas oryzae pv. oryzae. One of the transgenic rice lines was highly resistant to Xanthomonas oryzae pv. oryzae. Interestingly, this line also showed remarkably high resistance to Magnaporthe grisea, the fungal pathogen causing rice blast, which is the most devastating rice disease in many countries. The causal rice gene, encoding a putative receptor‐like cytoplasmic kinase, was therefore designated as BROAD‐SPECTRUM RESISTANCE 1. Our results demonstrate the utility of the rice‐FOX Arabidopsis lines as a tool for the identification of genes involved in plant defence and suggest the presence of a defence mechanism common between monocots and dicots.
ISSN:1467-7644
1467-7652
DOI:10.1111/j.1467-7652.2010.00568.x