β‐Carotene and its cleavage enzyme β‐carotene‐15,15′‐oxygenase (CMOI) affect retinoid metabolism in developing tissues

ABSTRACT The mammalian embryo relies on maternal circulating retinoids (vitamin A derivatives) for development. β‐Carotene is the major human dietary provitamin A. β‐Carotene‐15,15′‐oxygenase (CMOI) has been proposed as the main enzyme generating retinoid from β‐carotene in vivo. CMOI is expressed i...

Full description

Saved in:
Bibliographic Details
Published in:The FASEB journal 2011-05, Vol.25 (5), p.1641-1652
Main Authors: Kim, Youn-Kyung, Wassef, Lesley, Chung, Stacey, Jiang, Hongfeng, Wyss, Adrian, Blaner, William S., Quadro, Loredana
Format: Article
Language:English
Subjects:
Acyltransferases - metabolism
Animals
beta Carotene - metabolism
beta-Carotene 15,15'-Monooxygenase - genetics
beta-Carotene 15,15'-Monooxygenase - metabolism
Blotting, Western
Chromatography, High Pressure Liquid
Embryo, Mammalian - enzymology
Embryo, Mammalian - metabolism
embryonic vitamin A deficiency
Female
lecithin:retinol acyltransferase
LRAT
Mice
Mice, Knockout
Pregnancy
provitamin A
Research Communications
Retinoids - metabolism
Retinol-Binding Proteins - genetics
Retinol-Binding Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tretinoin - metabolism
Vitamin A - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT The mammalian embryo relies on maternal circulating retinoids (vitamin A derivatives) for development. β‐Carotene is the major human dietary provitamin A. β‐Carotene‐15,15′‐oxygenase (CMOI) has been proposed as the main enzyme generating retinoid from β‐carotene in vivo. CMOI is expressed in embryonic tissues, suggesting that β‐carotene provides retinoids locally during development. We performed loss of CMOI function studies in mice lacking retinol‐binding protein (RBP), an established model of embryonic vitamin A deficiency (VAD). We show that, unexpectedly, lack of CMOI in the developing tissues further exacerbates the severity of VAD and thus the embryonic malformations of RBP−/− mice. Since β ‐carotene was not present in any of the mouse diets, we unveiled a novel action of CMOI independent from its β ‐carotene cleavage activity. We also show for the first time that CMOI exerts an additional function on retinoid metabolism by influencing retinyl ester formation via modulation of lecithin:retinol acyltransferase (LRAT) activity, at least in developing tissues. Finally, we demonstrate unequivocally that β‐carotene can serve as an alternative vitamin A source for the in situ synthesis of retinoids in developing tissues by the action of CMOI.—Kim, Y.‐K., Wassef, L., Chung, S., Jiang, H., Wyss, A., Blaner, W. S., Quadro, L. β‐Carotene and its cleavage enzyme β‐carotene‐15,15′‐oxygenase (CMOI) affect retinoid metabolism in developing tissues. FASEB J. 25, 1641–1652 (2011). www.fasebj.org
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.10-175448