Structural characterization of d(CAACCCGTTG) and d(CAACGGGTTG) mini-hairpin loops by heteronuclear NMR: the effects of purines versus pyrimidines in DNA hairpins

ABSTRACT The DNA decamers, d(CAACCCGTTG) and d(CAACGGGTTG) were studied in solution by proton and heteronuclear NMR. Under appropriate conditions of pH, temperature, salt concentration and DNA concentration, both decamers form hairpin conformations with similar stabilities [Avizonis and Kearns (1995...

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Bibliographic Details
Published in:Nucleic acids research 1995-04, Vol.23 (7), p.1260-1268
Main Authors: Avizonis, Daina Z., Kearns, David R.
Format: Article
Language:English
Subjects:
Base Sequence
Carbohydrates - chemistry
Deoxyribonucleotides - chemistry
DNA - chemistry
Magnetic Resonance Spectroscopy - methods
Models, Molecular
Molecular Sequence Data
Molecular Structure
Nucleic Acid Conformation
Protons
Purines - chemistry
Pyrimidines - chemistry
Thermodynamics
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Summary:ABSTRACT The DNA decamers, d(CAACCCGTTG) and d(CAACGGGTTG) were studied in solution by proton and heteronuclear NMR. Under appropriate conditions of pH, temperature, salt concentration and DNA concentration, both decamers form hairpin conformations with similar stabilities [Avizonis and Kearns (1995) Biopolymers, 35, 187–200]. Both decamers adoptmini-hairpin loops, where the first and last four nucleotides are involved in Watson-Crick hydrogen bonding and the central two nucleotides, CC or GG respectively, form the loop. Through the use of proton-proton, proton-phosphorus and natural abundance proton-carbon NMR experiments, backbone torsion angles (β, γ and ε), sugar puckers and interproton distances were measured. The nucleotides forming the loops of these decamers were found to stack upon one another in an L1 type of loop conformation. Both show γtr and unusual β torsion angles in the loop-closingnucleotide G7 as expected for mini-hairpin loop formation. Our results indicate that the β and ε torsion angles of the fifth and sixth nucleotides that form the loop and the loop-closing nucleotide G7 are notin the standard trans conformation as found in B-DNA. Although the loop structures calculated from NMR-derived constraints are not well defined, the stacking of the bases in the two different hairpins is different. This difference in the base stacking of the loop may provide an explanation as to why the cytosine-containing hairpin is thermodynamically more stable than the guanine-containing hairpin.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/23.7.1260