Irreversible electroporation of the liver and liver hilum in swine

Abstract Background Irreversible electroporation (IRE) is a novel, non-thermal form of ablation. We studied the safety and efficacy of IRE for the ablation of liver tissue around the liver hilum. We also studied the ability of triphenyltetrazolium chloride staining (TTC) to predict the zone of ablat...

Full description

Saved in:
Bibliographic Details
Published in:HPB (Oxford, England) England), 2011-03, Vol.13 (3), p.168-173
Main Authors: Charpentier, Kevin P, Wolf, Farrah, Noble, Lelia, Winn, Brody, Resnick, Murray, Dupuy, Damian E
Format: Article
Language:English
Subjects:
ablation
Animals
Catheter Ablation - adverse effects
Catheter Ablation - methods
Coloring Agents
electroporation
Electroporation - methods
Female
Gastroenterology and Hepatology
Laparotomy
liver
Liver - surgery
Models, Animal
Original
Predictive Value of Tests
Sus scrofa
Tetrazolium Salts
tumour
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background Irreversible electroporation (IRE) is a novel, non-thermal form of ablation. We studied the safety and efficacy of IRE for the ablation of liver tissue around the liver hilum. We also studied the ability of triphenyltetrazolium chloride staining (TTC) to predict the zone of ablation after IRE. Methods Eight swine underwent 20 ablations of the liver and liver hilum. Two monopolar probes were positioned 2 cm apart. IRE was performed using 90 pulses of 2500–3000 V/cm. IRE treatments were performed from 15 min to 14 days ( n = 4) before sacrifice. Results All animals survived. No major complications were encountered. Ablation width ranged from 2.27 to 4.45 cm and ablation height ranged from 1.5 to 1.8 cm. TTC staining demonstrated the zone of ablation in all animals. Hepatocyte necrosis occurs immediately adjacent to large central veins without evidence of heat sink. Bile ducts, portal veins and hepatic arteries appear to be more resistant to the effects of IRE. Conclusions IRE appears to be safe and effective for liver tissue ablation in the liver hilum. The portal structures appear more resistant to the effects of IRE. TTC staining can predict the zone of IRE ablation as early as 15 min after treatment.
ISSN:1365-182X
1477-2574
DOI:10.1111/j.1477-2574.2010.00261.x