Visualization of interaction between inorganic nanoparticles and bacteria or fungi

The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility o...

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Bibliographic Details
Published in:International journal of nanomedicine 2010-01, Vol.5, p.1085-1094
Main Authors: Chwalibog, André, Sawosz, Ewa, Hotowy, Anna, Szeliga, Jacek, Mitura, Stanislaw, Mitura, Katarzyna, Grodzik, Marta, Orlowski, Piotr, Sokolowska, Aleksandra
Format: Article
Language:English
Subjects:
Bacteria
Candida albicans
Candida albicans - chemistry
Candida albicans - cytology
Candida albicans - drug effects
Candida albicans - metabolism
Colloids - chemistry
Colloids - pharmacology
diamond
Diamond - chemistry
Diamond - pharmacology
Drug Delivery Systems
Fungi
gold
Gold - chemistry
Gold - pharmacology
Microorganisms
morphology
Nanocomposites - chemistry
Nanoparticles
Nanoparticles - chemistry
Original Research
platinum
Platinum - chemistry
Platinum - pharmacology
silver
Silver - chemistry
Silver - pharmacology
Staphylococcus aureus
Staphylococcus aureus - chemistry
Staphylococcus aureus - cytology
Staphylococcus aureus - drug effects
Staphylococcus aureus - metabolism
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Summary:The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a "noncontact" interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/ijn.s13532