Real‐Time SNP Analysis in Secondary‐Structure‐Folded Nucleic Acids

A tricomponent sensor was designed for genotyping stem‐loop‐structured DNA sequences. DNA adaptor strand f (see picture; red) hybridizes to the analyte (orange) with high affinity and unwinds its secondary structure; strand m (green) forms a stable complex only with the fully complementary analyte s...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2010-11, Vol.49 (47), p.8950-8953
Main Authors: Grimes, Jeffrey, Gerasimova, Yulia V, Kolpashchikov, Dmitry M
Format: Article
Language:English
Subjects:
biotransformations
DNA - chemistry
DNA Probes - chemistry
Fluorescent Dyes - chemistry
genotyping
molecular beacons
Polymorphism, Single Nucleotide
real-time detection
Spectrometry, Fluorescence
tau proteins
tau Proteins - metabolism
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Summary:A tricomponent sensor was designed for genotyping stem‐loop‐structured DNA sequences. DNA adaptor strand f (see picture; red) hybridizes to the analyte (orange) with high affinity and unwinds its secondary structure; strand m (green) forms a stable complex only with the fully complementary analyte sequence. A fluorescing molecular beacon reports complex formation.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201004475