Expression and RNA Interference of Salivary Polygalacturonase Genes in the Tarnished Plant Bug, Lygus lineolaris

Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three tran...

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Bibliographic Details
Published in:Journal of insect science (Tucson, Ariz.) Ariz.), 2010, Vol.10 (173), p.1-13
Main Authors: Walker, William B., Allen, Margaret L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Diet
Enzymes
extraoral digestion
Gene expression
Gene Expression Profiling
gene knockdown
Genetic aspects
Gossypium
Hemiptera
Heteroptera - genetics
Heteroptera - metabolism
Mirids
Molecular Sequence Data
Physiological aspects
Polygalacturonase - genetics
Polygalacturonase - metabolism
Polymerase Chain Reaction
Properties
RNA Interference
RNAi
Saliva
salivary enzyme
Salivary glands
Salivary Glands - enzymology
secretions
Sequence Analysis, DNA
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Summary:Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris.
ISSN:1536-2442
1536-2442
DOI:10.1673/031.010.14133