Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing material does not have adverse effects on the conformation or functionality of membrane-bound molecules. In t...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2010-01, Vol.1 (6), p.688-696
Main Authors: Im, Hyungsoon, Wittenberg, Nathan J, Lesuffleur, Antoine, Lindquist, Nathan C, Oh, Sang-Hyun
Format: Article
Language:English
Subjects:
Antibodies
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Summary:Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing material does not have adverse effects on the conformation or functionality of membrane-bound molecules. In this work, pore-spanning lipid membranes are formed over an array of periodic nanopores in free-standing gold films for surface plasmon resonance (SPR) kinetic binding assays. The ability to perform kinetic assays with a transmembrane protein is demonstrated with α-hemolysin (α-HL). The incorporation of α-HL into the membrane followed by specific antibody binding (anti-α-HL) red-shifts the plasmon resonance of the gold nanopore array, which is optically monitored in real time. Subsequent fluorescence imaging reveals that the antibodies primarily bind in nanopore regions, indicating that α-HL incorporation preferentially occurs into areas of pore-spanning lipid membranes.
ISSN:2041-6520
2041-6539
DOI:10.1039/c0sc00365d