Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs

A role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells throu...

Full description

Saved in:
Bibliographic Details
Published in:Blood 2010-12, Vol.116 (23), p.e118-e127
Main Authors: Jima, Dereje D., Zhang, Jenny, Jacobs, Cassandra, Richards, Kristy L., Dunphy, Cherie H., Choi, William W.L., Yan Au, Wing, Srivastava, Gopesh, Czader, Magdalena B., Rizzieri, David A., Lagoo, Anand S., Lugar, Patricia L., Mann, Karen P., Flowers, Christopher R., Bernal-Mizrachi, Leon, Naresh, Kikkeri N., Evens, Andrew M., Gordon, Leo I., Luftig, Micah, Friedman, Daphne R., Weinberg, J. Brice, Thompson, Michael A., Gill, Javed I., Liu, Qingquan, How, Tam, Grubor, Vladimir, Gao, Yuan, Patel, Amee, Wu, Han, Zhu, Jun, Blobe, Gerard C., Lipsky, Peter E., Chadburn, Amy, Dave, Sandeep S.
Format: Article
Language:English
Subjects:
B-Lymphocytes
Base Sequence
Chromatin Immunoprecipitation
Gene Expression Profiling - methods
Gene Library
High-Throughput Nucleotide Sequencing
Humans
Lymphoid Neoplasia
Lymphoma, Large B-Cell, Diffuse - genetics
MicroRNAs - analysis
MicroRNAs - genetics
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, RNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells through deep sequencing of 31 normal and malignant human B-cell samples that comprise the spectrum of B-cell differentiation and common malignant phenotypes. We identified the expression of 333 known miRNAs, which is more than twice the number previously recognized in any tissue type. We further identified the expression of 286 candidate novel miRNAs in normal and malignant B cells. These miRNAs were validated at a high rate (92%) using quantitative polymerase chain reaction, and we demonstrated their application in the distinction of clinically relevant subgroups of lymphoma. We further demonstrated that a novel miRNA cluster, previously annotated as a hypothetical gene LOC100130622, contains 6 novel miRNAs that regulate the transforming growth factor-β pathway. Thus, our work suggests that more than a third of the miRNAs present in most cellular types are currently unknown and that these miRNAs may regulate important cellular functions.
ISSN:0006-4971
1528-0020
1528-0020
DOI:10.1182/blood-2010-05-285403