The apical/basal-polarity determinant Scribble cooperates with the PCP core factor Stbm/Vang and functions as one of its effectors

Most tissues display several features of cellular polarization. Besides the ubiquitous epithelial polarization in the Apical–Basal (A/B) axis, many epithelia (and associated organs) display a Planar Cell Polarization (PCP). Recently, a crosstalk between the PCP and A/B polarity determinants has been...

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Bibliographic Details
Published in:Developmental biology 2009-09, Vol.333 (1), p.67-77
Main Authors: Courbard, Jean-Remy, Djiane, Alexandre, Wu, Jun, Mlodzik, Marek
Format: Article
Language:English
Subjects:
Animals
Apical–basal polarity
Body Patterning - physiology
Cell Polarity - physiology
Compound Eye, Arthropod - cytology
Compound Eye, Arthropod - growth & development
Compound Eye, Arthropod - metabolism
Drosophila
Drosophila - cytology
Drosophila - growth & development
Drosophila - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Epithelial patterning
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mutation
Planar cell polarity
Protein Binding
Van Gogh
Wings, Animal - cytology
Wings, Animal - growth & development
Wings, Animal - metabolism
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Summary:Most tissues display several features of cellular polarization. Besides the ubiquitous epithelial polarization in the Apical–Basal (A/B) axis, many epithelia (and associated organs) display a Planar Cell Polarization (PCP). Recently, a crosstalk between the PCP and A/B polarity determinants has been suggested, i.e. the activity or stability of the PCP factor Frizzled is regulated by the A/B determinants aPKC and Bazooka in the Drosophila eye. We have systematically investigated genetic and physical interactions between the Drosophila A/B factors and the core PCP component Strabismus (Stbm)/Van Gogh (Vang). The A/B determinant Scribble was found to interact both genetically and physically with Stbm/Vang. We demonstrate that Scribble binds Stbm/Vang through its PDZ domain 3 and that it cooperates with Stbm/Vang in PCP establishment. Our data indicate that Scribble, in addition to its role in A/B polarity, has a distinct requirement in PCP establishment in the Drosophila eye and wing. We define a scribble allele that is largely PCP specific. Our data show that Scribble is part of the Stbm/Vang PCP complex and further suggest that it might act as an effector of Stbm/Vang during PCP establishment.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2009.06.024