SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

An effective high-speed countercurrent chromatography (HSCCC) method was established for further separation and purification of four minor flavonols in addition to five major flavonols which were reported by our previous study from extracts of Flos gossypii. HSCCC was performed with three two-phase...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2010-01, Vol.33 (16), p.1502-1515
Main Authors: Yang, Yi, Zhao, Yongxin, Gu, Dongyu, Ayupbek, Amatjan, Huang, Yun, Dou, Jun, Ito, Yoichiro, Zhang, Tianyou, Aisa, Haji Akber
Format: Article
Language:English
Subjects:
Biological and medical sciences
flavonol
Flos gossypii
General pharmacology
HSCCC
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
separation rules of flavonols
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Summary:An effective high-speed countercurrent chromatography (HSCCC) method was established for further separation and purification of four minor flavonols in addition to five major flavonols which were reported by our previous study from extracts of Flos gossypii. HSCCC was performed with three two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (7.5:15:6:7, v/v), (2.5:15:2:7, v/v), and (0:1:0:1, v/v). The separation was repeated three times, and 3.8 mg of 8-methoxyl-kaempferol-7-O-β-D-rhamnoside (HPLC purity 98.27%), 6.7 mg of astragalin (HPLC purity 94.18%), 3.3 mg of 4′-methoxyl-quercetin-7-O-β-D-glucoside (HPLC purity 94.30%), and 8.2 mg of hyperoside (HPLC purity 93.48%) were separated from 150 mg of the crude sample. The chemical structures of the flavonols were confirmed by MS, 1 H NMR, and 13 C NMR. Meanwhile, the results indicated that the target compound with smaller K value (
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2010.489000