Intravascular pressure augments cerebral arterial constriction by inducing voltage‐insensitive Ca2+ waves

This study examined whether elevated intravascular pressure stimulates asynchronous Ca2+ waves in cerebral arterial smooth muscle cells and if their generation contributes to myogenic tone development. The endothelium was removed from rat cerebral arteries, which were then mounted in an arteriograph...

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Bibliographic Details
Published in:The Journal of physiology 2010-10, Vol.588 (20), p.3983-4005
Main Authors: Mufti, Rania E., Brett, Suzanne E., Tran, Cam Ha T., Abd El‐Rahman, Rasha, Anfinogenova, Yana, El‐Yazbi, Ahmed, Cole, William C., Jones, Peter P., Chen, S.R. Wayne, Welsh, Donald G.
Format: Article
Language:English
Subjects:
Angiography
Animals
Blotting, Western
Calcium Signaling - drug effects
Calcium Signaling - physiology
Cardiovascular
Cerebral Arteries - drug effects
Cerebral Arteries - physiology
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiology
Enzyme Inhibitors - pharmacology
Female
Membrane Potentials - drug effects
Membrane Potentials - physiology
Microscopy, Confocal
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - physiology
Myocytes, Smooth Muscle - drug effects
Myocytes, Smooth Muscle - physiology
Myography
Phosphatase
Phosphorylation
Phosphorylation - drug effects
Phosphorylation - physiology
Rats
Rats, Sprague-Dawley
Rodents
Ryanodine - pharmacology
Sarcoplasmic Reticulum - drug effects
Sarcoplasmic Reticulum - metabolism
Smooth muscle
Thapsigargin - pharmacology
Vasoconstriction - drug effects
Vasoconstriction - physiology
Veins & arteries
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Summary:This study examined whether elevated intravascular pressure stimulates asynchronous Ca2+ waves in cerebral arterial smooth muscle cells and if their generation contributes to myogenic tone development. The endothelium was removed from rat cerebral arteries, which were then mounted in an arteriograph, pressurized (20–100 mmHg) and examined under a variety of experimental conditions. Diameter and membrane potential (VM) were monitored using conventional techniques; Ca2+ wave generation and myosin light chain (MLC20)/MYPT1 (myosin phosphatase targeting subunit) phosphorylation were assessed by confocal microscopy and Western blot analysis, respectively. Elevating intravascular pressure increased the proportion of smooth muscle cells firing asynchronous Ca2+ waves as well as event frequency. Ca2+ wave augmentation occurred primarily at lower intravascular pressures (
ISSN:0022-3751
1469-7793
DOI:10.1113/jphysiol.2010.193300