Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice

Chitin is a major molecular pattern for various fungi, and its fragments, chitin oligosaccharides, are known to induce various defense responses in plant cells. A plasma membrane glycoprotein, CEBiP (chitin elicitor binding protein) and a receptor kinase, CERK1 (chitin elicitor receptor kinase) (als...

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Published in:The Plant journal : for cell and molecular biology 2010-10, Vol.64 (2), p.204-214
Main Authors: Shimizu, Takeo, Nakano, Takuto, Takamizawa, Daisuke, Desaki, Yoshitake, Ishii-Minami, Naoko, Nishizawa, Yoko, Minami, Eiichi, Okada, Kazunori, Yamane, Hisakazu, Kaku, Hanae, Shibuya, Naoto
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Arabidopsis
Biological and medical sciences
Carbohydrates
Cell physiology
Cell receptors
Cell structures and functions
Cellular biology
Chitin
Chitin - metabolism
chitin elicitor
Defense mechanisms
Fundamental and applied biological sciences. Psychology
Fungi
Gene Knockdown Techniques
Glycoproteins
Immunoprecipitation
LysM receptor
Miscellaneous
Molecular and cellular biology
Molecular biology
Molecular Sequence Data
oligosaccharides
Original
Oryza - enzymology
Oryza - genetics
Oryza sativa
Plant biology
Plant cells
Plant physiology and development
Plant Proteins - metabolism
Plasma membranes
Protein Multimerization
Protein-Serine-Threonine Kinases - metabolism
receptor complex
receptor-like kinase
Rice
Signal Transduction
Two-Hybrid System Techniques
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Summary:Chitin is a major molecular pattern for various fungi, and its fragments, chitin oligosaccharides, are known to induce various defense responses in plant cells. A plasma membrane glycoprotein, CEBiP (chitin elicitor binding protein) and a receptor kinase, CERK1 (chitin elicitor receptor kinase) (also known as LysM-RLK1), were identified as critical components for chitin signaling in rice and Arabidopsis, respectively. However, it is not known whether each plant species requires both of these two types of molecules for chitin signaling, nor the relationships between these molecules in membrane signaling. We report here that rice cells require a LysM receptor-like kinase, OsCERK1, in addition to CEBiP, for chitin signaling. Knockdown of OsCERK1 resulted in marked suppression of the defense responses induced by chitin oligosaccharides, indicating that OsCERK1 is essential for chitin signaling in rice. The results of a yeast two-hybrid assay indicated that both CEBiP and OsCERK1 have the potential to form hetero- or homo-oligomers. Immunoprecipitation using a membrane preparation from rice cells treated with chitin oligosaccharides suggested the ligand-induced formation of a receptor complex containing both CEBiP and OsCERK1. Blue native PAGE and chemical cross-linking experiments also suggested that a major portion of CEBiP exists as homo-oligomers even in the absence of chitin oligosaccharides.
ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313x.2010.04324.x