Stimulation of the Expression of Osteogenic and Chondrogenic Phenotypes in vitro by Osteogenin

Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understand the mechanism of action of osteogenin, we examined its influence on perioste...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-11, Vol.86 (22), p.8793-8797
Main Authors: Vukicevic, Slobodan, Luyten, Frank P., Reddi, A. H.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Biological and medical sciences
Bone marrow
Bone Marrow - drug effects
Bone Marrow Cells
Bone Matrix - physiology
Bone Morphogenetic Protein 3
Bone Morphogenetic Proteins
Bones
Cartilage
Cartilage - cytology
Cartilage - drug effects
Cartilage - metabolism
Cattle
Cell differentiation, maturation, development, hematopoiesis
Cell Division - drug effects
Cell physiology
Cells
Cells, Cultured
Chondrocytes
Cultured cells
Cyclic AMP - metabolism
DNA Replication - drug effects
Fetus
Fundamental and applied biological sciences. Psychology
Kinetics
Male
Molecular and cellular biology
Osteoblasts
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Parathyroid Hormone - pharmacology
Periosteum - cytology
Periosteum - drug effects
Periosteum - metabolism
Phenotype
Phenotypes
Phosphatases
Protein Biosynthesis
Proteins - isolation & purification
Proteins - pharmacology
Proteoglycans - biosynthesis
Rats
Rats, Inbred F344
Stromal cells
Sulfates - metabolism
Transforming Growth Factors - pharmacology
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Summary:Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understand the mechanism of action of osteogenin, we examined its influence on periosteal cells, osteoblasts, fibroblasts, chondrocytes, and bone marrow stromal cells in vitro. Osteogenin stimulated alkaline phosphatase activity and collagen synthesis in periosteal cells. The cAMP response to parathyroid hormone in periosteal cells was increased by osteogenin. In primary cultures of calvarial osteoblasts, osteogenin stimulated alkaline phosphatase activity, the cAMP response to parathyroid hormone, and the synthesis of collagenous and noncollagenous proteins; however, cell proliferation was not affected. Osteogenin increased the production of sulfated proteoglycans in fetal rat chondroblasts and in rabbit articular chondrocytes. The present experiments demonstrate the significant influence of osteogenin in the stimulation of osteogenic and chondrogenic phenotypes in vitro.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.22.8793