Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes

Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candid...

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Bibliographic Details
Published in:Developmental dynamics 2009-06, Vol.238 (6), p.1398-1346
Main Authors: Khokha, Mustafa K., Krylov, Vladimir, Reilly, Michael J., Gall, Joseph G., Bhattacharya, Dipankan, Cheung, Chung Yan J., Kaufman, Sarah, Lam, Dang Khoa, Macha, Jaroslav, Ngo, Catherine, Prakash, Neha, Schmidt, Philip, Tlapakova, Tereza, Trivedi, Toral, Tumova, Lucie, Abu‐Daya, Anita, Geach, Timothy, Vendrell, Elisenda, Ironfield, Holly, Sinzelle, Ludivine, Sater, Amy K., Wells, Dan E., Harland, Richard M., Zimmerman, Lyle B.
Format: Article
Language:English
Subjects:
Animals
centromere
Centromere - genetics
Chromosome Mapping - methods
Chromosomes
Female
Freshwater
Genetic Markers
genetics
interference
Male
mapping
Meiosis - physiology
Mutation
recombination
Recombination, Genetic
Xenopus - genetics
Xenopus tropicalis
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Summary:Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398–1406, 2009. © 2009 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.21965