Proteomic analyses of monocyte-derived macrophages infected with human immunodeficiency virus type 1 primary isolates from Hispanic women with and without cognitive impairment

The signature for human immunodeficiency virus type 1 (HIV-1) neurovirulence remains a subject of intense debate. Macrophage viral tropism is one prerequisite but others, including virus-induced alterations in innate and adaptive immunity, remain under investigation. HIV-1-infected mononuclear phago...

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Bibliographic Details
Published in:Journal of neurovirology 2009-01, Vol.15 (1), p.36-50
Main Authors: Toro-Nieves, Dm, Rodriguez, Y, Plaud, M, Ciborowski, P, Duan, F, Laspiur, J Pérez, Wojna, V, Meléndez, LM
Format: Article
Language:English
Subjects:
AIDS Dementia Complex - blood
AIDS Dementia Complex - metabolism
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cells, Cultured
Cognition
Cognition Disorders - blood
Cognition Disorders - metabolism
Cognition Disorders - virology
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Hispanic or Latino
HIV Infections - blood
HIV Infections - complications
HIV Infections - metabolism
HIV-1 - pathogenicity
HIV-1 - physiology
Human immunodeficiency virus 1
Human viral diseases
Humans
Immunology
Infectious Diseases
Macrophages - metabolism
Macrophages - virology
Medical sciences
Neurology
Neurosciences
Proteome
Proteomics
Tandem Mass Spectrometry
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral diseases of the nervous system
Virology
Virulence
Virus Replication
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Summary:The signature for human immunodeficiency virus type 1 (HIV-1) neurovirulence remains a subject of intense debate. Macrophage viral tropism is one prerequisite but others, including virus-induced alterations in innate and adaptive immunity, remain under investigation. HIV-1-infected mononuclear phagocytes (MPs; perivascular macrophages and microglia) secrete toxins that affect neurons. The authors hypothesize that neurovirulent HIV-1 variants affect the MP proteome by inducing a signature of neurotoxic proteins and thus affect cognitive function. To test this hypothesis, HIV-1 isolates obtained from peripheral blood of women with normal cognition (NC) were compared to isolates obtained from women with cognitive impairment (CI) and to the laboratory adapted SF162, a spinal fluid R5 isolate from a patient with HIV-1-associated dementia. HIV-1 isolates were used to infect monocyte-derived macrophages (MDMs) and infection monitored by secreted HIV-1 p24 by enzyme-linked immunosorbent assay (ELISA). Cell lysates of uninfected and HIV-1-infected MDMs at 14 days post infection were fractionated by cationic exchange chromatography and analyzed by surface enhanced laser desorption ionization time of flight (SELDI-TOF) using generalized estimating equations statistics. Proteins were separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE) and identified by tandem mass spectrometry. Levels of viral replication were similar amongst the HIV-1 isolates, although higher levels were obtained from one viral strain obtained from a patient with CI. Significant differences were found in protein profiles between virus-infected MDMs with NC, CI, and SF162 isolates (adjusted P value after multiple testing corrections, or q value
ISSN:1355-0284
1538-2443
DOI:10.1080/13550280802385505