Marrow grafts between canine siblings matched by serotyping and mixed leukocyte culture

Marrow grafts were carried out between 16 canine sibling donor-recipient pairs. The pairs were matched by serological histocompatibility testing and were nonreactive in a one-way mixed leukocyte culture. Recipients were prepared for transplantation by 1500-1580 R of total body irradiation. Donor mar...

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Bibliographic Details
Published in:The Journal of clinical investigation 1971-06, Vol.50 (6), p.1272-1275
Main Authors: Storb, R, Rudolph, R H, Thomas, E D
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Transplantation
Culture Techniques
Dogs - immunology
Graft vs Host Disease - etiology
Histocompatibility
Immunosuppression
Leukocyte Count
Leukocytes - immunology
Leukocytes - metabolism
Methotrexate - therapeutic use
Models, Biological
Serotyping
Transplantation, Homologous
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Summary:Marrow grafts were carried out between 16 canine sibling donor-recipient pairs. The pairs were matched by serological histocompatibility testing and were nonreactive in a one-way mixed leukocyte culture. Recipients were prepared for transplantation by 1500-1580 R of total body irradiation. Donor marrow was infused within 4 hr of irradiation. Recipients were not given immunosuppressive drug therapy after grafting. All 16 recipients showed evidence of prompt and sustained allogeneic marrow engraftment. Six died between 30 and 128 days after grafting with graft-versus-host disease, and three died between days 72 and 230 with pneumonia but no evidence of graft-versus-host disease with the exception of lymphoid atrophy. Seven recipients survived without graft-versus-host disease and are in excellent health between 200 and 684 after grafting. In summary, fatal graft-versus-host disease was observed in a number of canine recipients despite matching with their sibling donor by serological histocompatibility testing and by mixed leukocyte culture in a manner similar to that employed to define human HL-A matched sibling pairs. The graft-versus-host disease in these matched siblings developed more slowly than that observed in mismatched dogs, but the ultimate death of approximately half of the matched recipients emphasizes the need for posttransplantation immunosuppression even in this "compatible" situation.
ISSN:0021-9738
1558-8238
DOI:10.1172/JCI106605