MAL2 Selectively Regulates Polymeric IgA Receptor Delivery from the Golgi to the Plasma Membrane in WIF-B Cells

Myelin and lymphocyte protein 2 (MAL2) has been identified as a hepatic transcytotic regulator that mediates delivery from basolateral endosomes to the subapical compartment (SAC). However, overexpression of polymeric immunoglobulin A-receptor (pIgA-R) in polarized, hepatic WIF-B cells led to the dr...

Full description

Saved in:
Bibliographic Details
Published in:Traffic (Copenhagen, Denmark) Denmark), 2010-08, Vol.11 (8), p.1056-1066
Main Authors: In, Julie G, Tuma, Pamela L
Format: Article
Language:English
Subjects:
Animals
apical sorting
Cell Line
Cell Membrane - metabolism
Dipeptidyl Peptidase 4 - genetics
Dipeptidyl Peptidase 4 - metabolism
Golgi Apparatus - metabolism
Golgi Apparatus - ultrastructure
hepatocytes
Humans
MAL2
Membrane Proteins - genetics
Membrane Proteins - metabolism
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Myelin and Lymphocyte-Associated Proteolipid Proteins
Myelin Proteins - genetics
Myelin Proteins - metabolism
pIgA-R
Protein Isoforms - genetics
Protein Isoforms - metabolism
Proteolipids - genetics
Proteolipids - metabolism
Rats
Receptors, Fc - genetics
Receptors, Fc - metabolism
Receptors, Polymeric Immunoglobulin - genetics
Receptors, Polymeric Immunoglobulin - metabolism
WIF-B cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Myelin and lymphocyte protein 2 (MAL2) has been identified as a hepatic transcytotic regulator that mediates delivery from basolateral endosomes to the subapical compartment (SAC). However, overexpression of polymeric immunoglobulin A-receptor (pIgA-R) in polarized, hepatic WIF-B cells led to the dramatic redistribution of MAL2 into the Golgi and all the transcytotic intermediates occupied by the receptor. Although overexpressed hemagglutinin and dipeptidylpeptidase IV (DPPIV) distributed to the same compartments, MAL2 distributions did not change indicating the effect is selective. Cycloheximide treatment led to decreased pIgA-R and MAL2 intracellular staining, first in the Golgi then the SAC, suggesting they were apically delivered and that MAL2 was mediating the process. This was tested in Clone 9 cells (that lack endogenous MAL2). When expressed alone, pIgA-R was restricted to the Golgi whereas when coexpressed with MAL2, it distributed to the surface, was internalized and delivered to MAL2-positive puncta. In contrast, DPPIV distributions were independent of MAL2. Surface delivery of newly synthesized pIgA-R, but not DPPIV, was enhanced greater than ninefold by MAL2 coexpression. In WIF-B cells where MAL2 expression was knocked down, pIgA-R, but not DPPIV, was retained in the Golgi and its basolateral delivery was impaired. Thus, in addition to its role in transcytosis, MAL2 also regulates pIgA-R delivery from the Golgi to the plasma membrane.
ISSN:1398-9219
1600-0854
DOI:10.1111/j.1600-0854.2010.01074.x