Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum

To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit...

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Bibliographic Details
Published in:Molecular biology reports 2010-07, Vol.37 (6), p.2893-2902
Main Authors: Moyano, Eva M, González, Luis Miguel, Cuevas, Laureano, Perez-Pastrana, Esperanza, Santa-Maria, Ysmael, Benito, Agustín
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal Anatomy
Animal Biochemistry
Antigens, Protozoan - immunology
Biomedical and Life Sciences
Blotting, Western
Cell Extracts
Cloning
Cloning, Molecular
Computational Biology
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
electron microscopy
enzyme-linked immunosorbent assay
Expression library
Genetic Markers
Genetic Variation
Genetics
Histology
Humans
Life Sciences
Malaria
Molecular biology
Molecular Sequence Data
Morphology
Parasites
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
Plasmodium falciparum - ultrastructure
Protein Transport
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Protozoan Proteins - ultrastructure
Recombinant Fusion Proteins - metabolism
Sequence Alignment
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Summary:To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay's sensitivity and specificity were 78.2 and 94% respectively.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-009-9849-z