High-Throughput Lens-Free Blood Analysis on a Chip

We present a detailed investigation of the performance of lens-free holographic microscopy toward high-throughput on-chip blood analysis. Using a spatially incoherent source that is emanating from a large aperture, automated counting of red blood cells with minimal sample preparation steps at densit...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2010-06, Vol.82 (11), p.4621-4627
Main Authors: Seo, Sungkyu, Isikman, Serhan O, Sencan, Ikbal, Mudanyali, Onur, Su, Ting-Wei, Bishara, Waheb, Erlinger, Anthony, Ozcan, Aydogan
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Blood
Blood Cell Count - instrumentation
Blood Cell Count - methods
Cells
Chemistry
Erythrocyte Count
Erythrocytes
Exact sciences and technology
Global environmental pollution
Hemoglobin
Holography
Humans
Lab-On-A-Chip Devices
Lenses
Leukocyte Count
Microscopy
Pollution
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We present a detailed investigation of the performance of lens-free holographic microscopy toward high-throughput on-chip blood analysis. Using a spatially incoherent source that is emanating from a large aperture, automated counting of red blood cells with minimal sample preparation steps at densities reaching up to ∼0.4 × 106 cells/μL is presented. Using the same lens-free holographic microscopy platform, we also characterize the volume of the red blood cells at the single-cell level through recovery of the optical phase information of each cell. We further demonstrate the measurement of the hemoglobin concentration of whole blood samples as well as automated counting of white blood cells, also yielding spatial resolution at the subcellular level sufficient to differentiate granulocytes, monocytes, and lymphocytes from each other. These results uncover the prospects of lens-free holographic on-chip imaging to provide a useful tool for global health problems, especially by facilitating whole blood analysis in resource-poor environments.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac1007915