DC-SIGN (CD209) gene promoter polymorphisms in a Brazilian population and their association with human T-cell lymphotropic virus type 1 infection

1 Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil 2 School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil 3 Division of Clinical Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil 4 Department of Chemistry, Schoo...

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Published in:Journal of general virology 2009-04, Vol.90 (4), p.927-934
Main Authors: Kashima, Simone, Rodrigues, Evandra Strazza, Azevedo, Rochele, da Cruz Castelli, Erick, Mendes-Junior, Celso Teixeira, Yoshioka, France Keiko Nascimento, da Silva, Israel Tojal, Takayanagui, Osvaldo Massaiti, Covas, Dimas Tadeu
Format: Article
Language:English
Subjects:
Adult
Aged
Animal
Biological and medical sciences
Brazil - epidemiology
Brazil - ethnology
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - metabolism
Continental Population Groups - classification
Continental Population Groups - genetics
Female
Fundamental and applied biological sciences. Psychology
Genetic Predisposition to Disease
HTLV-I Infections - epidemiology
HTLV-I Infections - ethnology
HTLV-I Infections - genetics
Human T-lymphotropic virus
Human T-lymphotropic virus 1
Human T-lymphotropic virus 1 - pathogenicity
Humans
Lectins, C-Type - genetics
Lectins, C-Type - metabolism
Male
Microbiology
Middle Aged
Miscellaneous
Polymorphism, Single Nucleotide
Promoter Regions, Genetic - genetics
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Virology
Young Adult
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Summary:1 Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil 2 School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil 3 Division of Clinical Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil 4 Department of Chemistry, School of Philosophy, Sciences and Literature, University of São Paulo, Brazil 5 Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil Correspondence Dimas Tadeu Covas dimas{at}fmrp.usp.br This study evaluated four polymorphisms located in the DC-SIGN ( CD209 ) gene promoter region (positions –336, –332 –201 and –139) in DNA samples from four Brazilian ethnic groups (Caucasians, Afro-Brazilian, Asians and Amerindians) to establish the population distribution of these single-nucleotide polymorphisms (SNPs) and correlated DC-SIGN polymorphisms and infection in samples from human T-cell lymphotropic virus type 1 (HTLV-1)-infected individuals. To identify CD209 SNPs, 452 bp of the CD209 promoter region were sequenced and the genotype and allelic frequencies were evaluated. This is the first study to show genetic polymorphism in the CD209 gene in distinct Brazilian ethnic groups with the distribution of allelic and genotypic frequency. The results showed that –336A and –139A SNPs were quite common in Asians and that the –201T allele was not observed in Caucasians, Asians or Amerindians. No significant differences were observed between individuals with HTLV-1 disease and asymptomatic patients. However, the –336A variant was more frequent in HTLV-1-infected patients [HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), 80 %; healthy asymptomatic HTLV-1 carriers, 90 %] than in the control group (70 %) [ P =0.0197, odds ratio (OR)=2.511, 95 % confidence interval (CI)=1.218–5.179). In addition, the –139A allele was found to be associated with protection against HTLV-1 infection ( P =0.0037, OR=0.3758, 95 % CI=0.1954–0.7229) when the HTLV-1-infected patients as a whole were compared with the healthy-control group. These observations suggest that the –139A allele may be associated with HTLV-1 infection, although no significant association was observed among asymptomatic and HAM/TSP patients. In conclusion, the variation observed in SNPs –336 and –139 indicates that this lectin may be of crucial importance in the susceptibility/transmission of HTLV-1 infections. These authors contributed equally to this work.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.008367-0