Color and monochrome lensless on-chip imaging of Caenorhabditis elegans over a wide field-of-view

We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within 24 mm2. By digitally combining the reco...

Full description

Saved in:
Bibliographic Details
Published in:Lab on a chip 2010-01, Vol.10 (9), p.1109-1112
Main Authors: Isikman, Serhan O, Sencan, Ikbal, Mudanyali, Onur, Bishara, Waheb, Oztoprak, Cetin, Ozcan, Aydogan
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans - cytology
Colorimetry - instrumentation
Equipment Design
Equipment Failure Analysis
Holography - instrumentation
Image Enhancement - instrumentation
Lenses
Microfluidic Analytical Techniques - instrumentation
Microscopy - instrumentation
Reproducibility of Results
Sensitivity and Specificity
Signal Processing, Computer-Assisted - instrumentation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within 24 mm2. By digitally combining the reconstructed images at three different wavelengths (red, green and blue), color images of dyed samples are also acquired. This wide field-of-view and compact on-chip imaging modality also permits straightforward integration with microfluidic systems.
ISSN:1473-0197
1473-0189
DOI:10.1039/c001200a