Nuclear Matrix Association: Switching to the Invasive Cytotrophoblast

Abstract Abnormal trophoblast invasion is associated with the most common and most severe complications of human pregnancy. The biology of invasion, as well as the etiology of abnormal invasion remains poorly understood. The aim of this study was to characterize the transcriptome of the HTR-8/SVneo...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2010-05, Vol.31 (5), p.365-372
Main Authors: Drennan, K.J, Linnemann, A.K, Platts, A.E, Heng, H.H, Armant, D.R, Krawetz, S.A
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Blotting, Western
Cell Differentiation
Cells, Cultured
Comparative Genomic Hybridization
Embryology: invertebrates and vertebrates. Teratology
Epigenetics
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Silencing
Humans
Internal Medicine
Invasion
Matrix Attachment Regions - genetics
Nuclear matrix
Nuclear Matrix - genetics
Nuclear Matrix - metabolism
Obstetrics and Gynecology
Pregnancy
Pregnancy Trimester, First
Spectral Karyotyping
Trophoblast
Trophoblasts - cytology
Trophoblasts - metabolism
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Summary:Abstract Abnormal trophoblast invasion is associated with the most common and most severe complications of human pregnancy. The biology of invasion, as well as the etiology of abnormal invasion remains poorly understood. The aim of this study was to characterize the transcriptome of the HTR-8/SVneo human cytotrophoblast cell line which displays well characterized invasive and non-invasive behavior, and to correlate the activity of the transcriptome with nuclear matrix attachment and cell phenotype. Comparison of the invasive to non-invasive HTR transcriptomes was unremarkable. In contrast, comparison of the MARs on chromosomes 14–18 revealed an increased number of MARs associated with the invasive phenotype. These attachment areas were more likely to be associated with silent rather than actively transcribed genes. This study supports the view that nuclear matrix attachment may play an important role in cytotrophoblast invasion by ensuring specific silencing that facilitates invasion.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2010.02.012