Dysregulation of Promyelocytic Leukemia (PML) Protein Expression in Preeclamptic Placentae

Promyelocytic leukemia (PML) protein is a nucleoprotein that can regulate a variety of cellular stress responses. The aim of this study was to determine qualitative and quantitative changes in PML expression in preeclamptic placentae. Immunoblot, quantitative reverse transcription polymerase chain r...

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Bibliographic Details
Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2010-04, Vol.17 (4), p.339-349
Main Authors: Leavenworth, Jonathan D., Groesch, Kathleen A., Xin Hu, Malm, Scott, Torry, Ronald J., Abrams, Robert, Torry, Donald S.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Diseases of mother, fetus and pregnancy
Embryology
Female
Gynecology. Andrology. Obstetrics
Hematologic and hematopoietic diseases
Humans
Hypoxia - metabolism
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Medicine & Public Health
Nuclear Proteins - metabolism
Obstetrics/Perinatology/Midwifery
Original Article
Placenta - metabolism
Pre-Eclampsia - metabolism
Pregnancy
Pregnancy. Fetus. Placenta
Promyelocytic Leukemia Protein
Reproductive Medicine
Transcription Factors - metabolism
Trophoblasts - metabolism
Tumor Suppressor Proteins - metabolism
Young Adult
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Summary:Promyelocytic leukemia (PML) protein is a nucleoprotein that can regulate a variety of cellular stress responses. The aim of this study was to determine qualitative and quantitative changes in PML expression in preeclamptic placentae. Immunoblot, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and immunohistochemistry techniques were used to determine PML gene expression and localization in normal (n = 6) and preeclamptic (n = 6) placentae and primary cells. Promyelocytic leukemia protein was immunolocalized within nuclei of villus mesenchyme, but largely absent in trophoblast nuclei, with a trend for increased PML reactivity in preeclamptic placenta. Immunoblot analyses of nuclear extracts confirmed relative increases (∼3-fold) of PML expression in preeclamptic placentae (P < .05). Conversely, less PML messenger RNA (mRNA; ∼2-fold) was detected in preeclamptic versus normal placental samples. In vitro, PML expression could be increased by hypoxia in cultured endothelial cells but not trophoblast. Increased PML protein expression in preeclamptic villi suggests it could contribute to decreased vascularity and placental growth and/or function.
ISSN:1933-7191
1933-7205
DOI:10.1177/1933719109358455