Conserved nucleosome positioning defines replication origins

The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC bi...

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Bibliographic Details
Published in:Genes & development 2010-04, Vol.24 (8), p.748-753
Main Authors: Eaton, Matthew L, Galani, Kyriaki, Kang, Sukhyun, Bell, Stephen P, MacAlpine, David M
Format: Article
Language:English
Subjects:
Chromosomes, Fungal - genetics
Genome, Fungal - genetics
Nucleosomes - genetics
Replication Origin - genetics
Research Communication
Saccharomyces cerevisiae - genetics
Sequence Analysis
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Summary:The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1913210