Dynamics of Biological Macromolecules: Not a Simple Slaving by Hydration Water

We studied the dynamics of hydrated tRNA using neutron and dielectric spectroscopy techniques. A comparison of our results with earlier data reveals that the dynamics of hydrated tRNA is slower and varies more strongly with temperature than the dynamics of hydrated proteins. At the same time, tRNA a...

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Bibliographic Details
Published in:Biophysical journal 2010-04, Vol.98 (7), p.1321-1326
Main Authors: Khodadadi, S., Roh, J.H., Kisliuk, A., Mamontov, E., Tyagi, M., Woodson, S.A., Briber, R.M., Sokolov, A.P.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
BASIC BIOLOGICAL SCIENCES
Biochemistry
Biological
Biomolecules
Biophysics - methods
Deoxyribonucleic acid
Dielectrics
DNA
Dynamic tests
Dynamics
HYDRATION
Hydrogen - chemistry
Hydrogen Bonding
Macromolecular Substances
Macromolecules
Molecular structure
Muramidase - chemistry
Myoglobin - chemistry
NEUTRONS
Protein
PROTEINS
Ribonucleic acid
RNA
RNA, Transfer - chemistry
Scattering, Radiation
SPECTROSCOPY
Spectrum analysis
Temperature
TRANSFER RNA
Triticum - metabolism
WATER
Water - chemistry
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Summary:We studied the dynamics of hydrated tRNA using neutron and dielectric spectroscopy techniques. A comparison of our results with earlier data reveals that the dynamics of hydrated tRNA is slower and varies more strongly with temperature than the dynamics of hydrated proteins. At the same time, tRNA appears to have faster dynamics than DNA. We demonstrate that a similar difference appears in the dynamics of hydration water for these biomolecules. The results and analysis contradict the traditional view of slaved dynamics, which assumes that the dynamics of biological macromolecules just follows the dynamics of hydration water. Our results demonstrate that the dynamics of biological macromolecules and their hydration water depends strongly on the chemical and three-dimensional structures of the biomolecules. We conclude that the whole concept of slaving dynamics should be reconsidered, and that the mutual influence of biomolecules and their hydration water must be taken into account.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2009.12.4284