CagA C-terminal variations in Helicobacter pylori strains from Colombian patients with gastric precancerous lesions

The C-terminus of the Helicobacter pylori CagA protein is polymorphic, bearing different EPIYA sequences (EPIYA-A, B, C or D), and one or more CagA multimerization (CM) motifs. The number of EPIYA-C motifs is associated with precancerous lesions and gastric cancer (GC). The relationship between EPIY...

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Published in:Clinical microbiology and infection 2010-04, Vol.16 (4), p.369-378
Main Authors: Sicinschi, L.A., Correa, P., Peek, R.M., Camargo, M.C., Piazuelo, M.B., Romero-Gallo, J., Hobbs, S.S., Krishna, U., Delgado, A., Mera, R., Bravo, L.E., Schneider, B.G.
Format: Article
Language:English
Subjects:
Adult
Amino Acid Sequence
Antigens, Bacterial - chemistry
Antigens, Bacterial - genetics
Antigens, Bacterial - metabolism
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Biopsy
C-Terminus
CagA
cagA 3'region
CagA multimerization motif
CagA protein
Colombia
DNA
Dysplasia
EPIYA
ESS
Gastric cancer
Gastric Mucosa - metabolism
Gastric Mucosa - microbiology
Gastritis
genomics
Guanylate cyclase
H. pylori
H. pylori
Helicobacter Infections - complications
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Helicobacter pylori - metabolism
Human bacterial diseases
Humans
Infectious diseases
Intestine
Male
Medical sciences
Metaplasia
Middle Aged
Molecular Sequence Data
Polymorphism, Genetic
Precancerous Conditions - metabolism
Precancerous Conditions - microbiology
Precancerous Conditions - pathology
Risk factors
Risk groups
SHP-2 protein
Stomach Neoplasms - metabolism
Stomach Neoplasms - microbiology
Stomach Neoplasms - pathology
WSS
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Summary:The C-terminus of the Helicobacter pylori CagA protein is polymorphic, bearing different EPIYA sequences (EPIYA-A, B, C or D), and one or more CagA multimerization (CM) motifs. The number of EPIYA-C motifs is associated with precancerous lesions and gastric cancer (GC). The relationship between EPIYA, CM motifs and gastric lesions was examined in H. pylori-infected Colombian patients from areas of high and low risk for GC. Genomic DNA was extracted from H. pylori strains cultured from gastric biopsies from 80 adults with dyspeptic symptoms. Sixty-seven (83.8%) of 80 strains were cagA positive. The 3’ region of cagA was sequenced, and EPIYA and CM motifs were identified. CagA proteins contained one (64.2%), two (34.3%) or three EPIYA-C motifs (1.5%), all with Western type CagA-specific sequences. Strains with one EPIYA-C motif were associated with less severe gastric lesions (non-atrophic and multifocal atrophic gastritis), whereas strains with multiple EPIYA-C motifs were associated with more severe lesions (intestinal metaplasia and dysplasia) (p
ISSN:1198-743X
1469-0691
DOI:10.1111/j.1469-0691.2009.02811.x